FastQCFastQC Report
Wed 5 Aug 2015
trimmedgly5a.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmedgly5a.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97349
Sequences flagged as poor quality0
Sequence length36-50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT28592.9368560539913093No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG24182.4838467780870888No Hit
CAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACG18761.9270870784497014No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG16721.7175317671470687No Hit
CGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTA14591.4987313685810846No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT13321.3682729149760142No Hit
CGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGA11321.162826531345982No Hit
CTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGG10911.1207100227018254No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG8940.9183453348262437No Hit
CAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGG7980.8197310706838283No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC7780.7991864323208251No Hit
CGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCG7420.7622060832674192No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG6160.632774861580499No Hit
GAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCC6130.6296931658260485No Hit
CGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACC6090.6255842381534479No Hit
GAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCAC6040.620448078562697No Hit
GGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGAT5810.5968217444452434No Hit
AGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGC5370.5516235400466364No Hit
CTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAG5330.5475146123740356No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG4670.4797173057761251No Hit
GTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGAT4490.46122713124942216No Hit
CGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCT4450.4571182035768216No Hit
CGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTA4240.43554633329566816No Hit
CCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCG4210.43246463754121767No Hit
GGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTT4210.43246463754121767No Hit
AGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACC4190.43041017370491735No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA4170.42835570986861704No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG4110.42219231835971605No Hit
ATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGT4080.41911062260526555No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC4030.4139744630145148No Hit
CTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCT3990.40986553534191417No Hit
CCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCC3810.3913753608152113No Hit
CCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGG3800.39034812889706105No Hit
CTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTC3710.3811030416337096No Hit
TCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGA3600.36980349053405787No Hit
CTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCGAGGAA3580.36774902669775755No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC3470.3564494755981058No Hit
CGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTT3370.3461771564166042No Hit
CAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACAT3270.33590483723510256No Hit
CGGGAATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCG3230.33179590956250193No Hit
CTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGC3190.3276869818899013No Hit
CACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGC2620.26913476255534213No Hit
CACGGTTCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCT2620.26913476255534213No Hit
TCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAG2510.2578352114556903No Hit
TGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTA2500.2568079795375402No Hit
GCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTT2480.2547535157012399No Hit
GTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGG2400.24653566035603858No Hit
TCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGT2400.24653566035603858No Hit
ATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATC2340.24037226884713764No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT2250.23112718158378617No Hit
GTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCT2180.22393655815673505No Hit
GCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCC2140.2198276304841344No Hit
GCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCC2040.20955531130263277No Hit
GCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCG2000.20544638363003215No Hit
GGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTC1990.204419151711882No Hit
CTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATA1960.2013374559574315No Hit
AGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCT1910.19620129636668068No Hit
GCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCG1890.1941468325303804No Hit
CGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCA1840.1890106729396296No Hit
CGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCG1790.18387451334887878No Hit
AGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCA1750.17976558567627815No Hit
CTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTG1730.1777111218399778No Hit
GGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCG1640.16846603457662637No Hit
GTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAA1580.1623026430677254No Hit
CAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGC1550.1592209473132749No Hit
CCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGAC1550.1592209473132749No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGC1530.1571664834769746No Hit
ATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCC1500.15408478772252412No Hit
CAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGT1450.14894862813177331No Hit
GCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGATG1450.14894862813177331No Hit
CGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGATGCCATCTGCT1420.14586693237732282No Hit
CCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCG1410.14483970045917266No Hit
CGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCCACGATA1350.1386763089502717No Hit
GGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAG1310.13456738127767107No Hit
GTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCA1300.13354014935952088No Hit
GTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTC1290.13251291744137075No Hit
GTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTG1280.13148568552322057No Hit
CGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTA1280.13148568552322057No Hit
TCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGAC1260.12943122168692026No Hit
GTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGA1210.12429506209616945No Hit
TTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGA1200.12326783017801929No Hit
ATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTT1180.12121336634171896No Hit
CTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGC1180.12121336634171896No Hit
CGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCTGT1150.11813167058726849No Hit
GCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGA1150.11813167058726849No Hit
CGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAATTG1120.11504997483281802No Hit
GGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCAC1110.11402274291466784No Hit
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCG1100.1129955109965177No Hit
TACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCG1100.1129955109965177No Hit
AAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATC1080.11094104716021737No Hit
ACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGA1080.11094104716021737No Hit
CGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTC1070.1099138152420672No Hit
TTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGA1060.10888658332391704No Hit
CGCGAACTAGCCGAGATGACGATCCCAGTCGCTGTGGTTGTCCCGAGGAG1060.10888658332391704No Hit
GAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCA1060.10888658332391704No Hit
GGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCC1040.10683211948761671No Hit
GGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGG1040.10683211948761671No Hit
CTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCTGTGGTTG1030.10580488756946657No Hit
CGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCT1020.10477765565131639No Hit
GTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTAC980.10066872797871575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAA455.11136E-1043.632585
CGGGAAT558.367351E-1139.665981
GAATCGA608.518327E-932.7244344
GGGAATC608.518327E-932.7244342
ATCGAAC608.518327E-932.7244346
CTCGGTT4100.031.3941751
TCGAACC651.8579158E-830.2071697
TCGGTTA4300.029.9339792
CGGTTAC4550.028.2892553
TAAGCGA1004.7293724E-1126.8983138
CTAAGCG1004.7293724E-1126.82748837
GGTTACC4850.026.5394044
TTACCAA4900.026.2685956
CTCGCGT757.456583E-826.1795481
ACGGCTA1056.548362E-1126.15602744
GTTACCA4950.026.0032545
AAGCGAC1058.0035534E-1125.75341439
TACCAAG5050.025.4883377
CGTAGGG701.198965E-624.9329055
CCTAAGC1101.6734703E-1024.29905536
Produced by FastQC (version 0.11.3)