FastQCFastQC Report
Wed 5 Aug 2015
trimmedgly5b.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmedgly5b.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97391
Sequences flagged as poor quality0
Sequence length36-50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT29903.070098879773285No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG26372.7076423899538975No Hit
CAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACG22422.3020607653684633No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG17871.8348718054029631No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT14001.437504492201538No Hit
CGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGA12921.3266112885174195No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC10701.0986641476111756No Hit
CGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTA10551.0832623137661592No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG10351.062726535306137No Hit
CTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGG9761.0021459888490725No Hit
CAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGG7900.8111632491708679No Hit
GGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGAT7410.7608505919438141No Hit
CCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGG7240.7433951802527954No Hit
CGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCG6910.7095111457937592No Hit
GAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCAC6890.707457567947757No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG6450.6622788553357086No Hit
GAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCC6160.6325019765686768No Hit
CTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAG6000.6160733538006592No Hit
GGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTT5510.5657606965736054No Hit
CGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACC5400.5544660184205933No Hit
CGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCT5010.5144212504235505No Hit
AGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGC4630.47540327134950866No Hit
CGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTA4540.46616217104249885No Hit
GTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGAT4270.43843887012146915No Hit
TCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGA4150.4261174030454559No Hit
CCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCG4110.42201024735345155No Hit
CTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTC4070.4179030916614471No Hit
CCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCC3960.40660841350843513No Hit
CTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCT3930.4035280467394318No Hit
AGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACC3900.40044767997042846No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA3820.3922333685864197No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG3580.3675904344343933No Hit
GTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGG3500.3593761230503845No Hit
CGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTT3430.35218860058937684No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC3360.34500107812836917No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC3330.34192071135936586No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG3310.3398671335133636No Hit
CTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCGAGGAA3040.31214383259233397No Hit
ATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATC2840.291608054132312No Hit
GTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAA2810.28852768736330875No Hit
CACGGTTCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCT2810.28852768736330875No Hit
GCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTT2790.2864741095173065No Hit
GCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCG2770.2844205316713043No Hit
GCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCC2770.2844205316713043No Hit
AGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCT2600.26696511998028566No Hit
CGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCA2530.2597775975192779No Hit
CACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGC2530.2597775975192779No Hit
GGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTC2510.2577240196732758No Hit
ATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGT2500.2566972307502747No Hit
CAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACAT2470.25361686398127137No Hit
TCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAG2440.2505364972122681No Hit
GTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCT2360.24232218582825926No Hit
CGGGAATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCG2350.2412953969052582No Hit
TGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTA2330.23924181905925598No Hit
CTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGC2330.23924181905925598No Hit
GCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCG2170.22281319629123839No Hit
TCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGT2160.22178640736823732No Hit
CGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGGT2070.2125453070612274No Hit
CTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATA2030.20843815136922303No Hit
GGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAG1960.20125062890821535No Hit
GGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCG1910.19611668429320986No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT1890.19406310644720765No Hit
GGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCC1860.19098273967820434No Hit
CGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCG1850.18995595075520327No Hit
GTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTG1850.18995595075520327No Hit
GCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCC1820.18687558398619997No Hit
CTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTG1800.18482200614019775No Hit
GCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGATG1710.17558090583318786No Hit
CCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGAC1700.17455411691018677No Hit
CGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGATGCCATCTGCT1690.17352732798718568No Hit
ATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCC1650.16942017229518128No Hit
CGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAATTG1640.16839338337218018No Hit
GTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTC1620.166339805526178No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGC1610.16531301660317688No Hit
ACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGA1530.15709870521916808No Hit
AGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCA1510.15504512737316592No Hit
TACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCG1500.1540183384501648No Hit
TTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGA1430.14683081598915712No Hit
GTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGG1400.14375044922015381No Hit
CGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAAT1380.14169687137415163No Hit
GTTCCCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTG1370.1406700824511505No Hit
CAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGT1330.13656292675914614No Hit
GTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCA1330.13656292675914614No Hit
CGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCCACGATA1320.13553613783614502No Hit
CGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTC1290.1324557710671417No Hit
CGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCTGT1250.12834861537513734No Hit
CGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCG1250.12834861537513734No Hit
GCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAG1220.12526824860613406No Hit
GTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGA1170.12013430399112854No Hit
CCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCA1150.11808072614512635No Hit
CTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGC1120.11500035937612305No Hit
GTTTGCCTAGCCACCCCGGTCACCCGGAATGCTGGTGCGCTCTTACCGCA1120.11500035937612305No Hit
TCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGAC1110.11397357045312197No Hit
GGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCAC1100.11294678153012086No Hit
TTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGA1090.11191999260711975No Hit
GTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCA1080.11089320368411866No Hit
CCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCG1080.11089320368411866No Hit
GGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGG1060.10883962583811645No Hit
CAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGC1040.10678604799211426No Hit
CGCGAACTAGCCGAGATGACGATCCCAGTCGCTGTGGTTGTCCCGAGGAG1010.10370568122311095No Hit
GGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCAT1010.10370568122311095No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG980.10062531445410768No Hit
GAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCA980.10062531445410768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT254.5691944E-543.741141
GAATCGA357.5201424E-637.4924054
AATCGAA301.338017E-436.4509475
CCGCCTT250.002411003234.9929126
CTCGGTT4050.033.4808731
TCGGTTA4050.032.940862
ACGGTTC401.8772987E-532.8058552
CACGGTT401.8772987E-532.8058551
GGGAATC353.308875E-431.2436712
CGGTTAC4450.029.9798833
GGTTACC4650.028.6904244
CGTAGGG703.727473E-828.1193055
GTAGGGC703.727473E-828.1193056
GCGTAGG703.727473E-828.1193054
TAGGGCT703.727473E-828.1193057
CTCGCGT703.727473E-828.1193051
TTACCAA4850.027.5073176
GTTACCA4900.027.2266275
ACTTCGC1053.6379788E-1227.0778473
TACCAAG5000.026.6820957
Produced by FastQC (version 0.11.3)