FastQCFastQC Report
Wed 5 Aug 2015
trimmedgly7a.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmedgly7a.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96867
Sequences flagged as poor quality0
Sequence length36-50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTA17731.8303446994332437No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG15181.5670971538294776No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT10381.0715723621047415No Hit
CAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACG8200.8465215191964239No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT7650.789742636811298No Hit
CGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCG7650.789742636811298No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG6810.7030257982594692No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG6720.6937347084146304No Hit
GTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGAT6400.660699722299648No Hit
CGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGA5670.5853386602248444No Hit
CGGGAATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCG5490.5667564805351668No Hit
CGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTA5090.5254627478914388No Hit
CGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACC4990.5151393147305068No Hit
CTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCGAGGAA4890.5048158815695748No Hit
ATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGT4540.46868386550631275No Hit
CTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCT3920.4046785799085344No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG3640.3757729670579248No Hit
GAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCAC3420.35306141410387437No Hit
CAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGG3330.3437703242590356No Hit
CAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACAT3070.3169293980406124No Hit
CTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAG3060.3158970547245192No Hit
CTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGG3050.314864711408426No Hit
GGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGAT3040.3138323680923328No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG2900.299379561667028No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC2880.2973148750348416No Hit
GAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCC2690.2777003520290708No Hit
CCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCC2680.2766680087129776No Hit
GTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCT2470.25498879907502037No Hit
GCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCC2460.25395645575892717No Hit
CTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGC2460.25395645575892717No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA2370.24466536591408838No Hit
ATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATC2280.23537427606924957No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG2250.23227724612097No Hit
CCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGG2220.22918021617269038No Hit
TCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGA2130.2198891263278516No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC2080.2147274097473856No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT2070.2136950664312924No Hit
GCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCG2060.21266272311519918No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC2040.21059803648301278No Hit
ATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTT2020.20853334985082636No Hit
GGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTT1840.1899511701611488No Hit
CGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCG1840.1899511701611488No Hit
CCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCG1770.1827247669484964No Hit
CTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTC1670.1724013337875644No Hit
CCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCG1670.1724013337875644No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGC1660.1713689904714712No Hit
GTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTC1620.1672396172070984No Hit
AGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGC1610.1662072738910052No Hit
AGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACC1560.16104555731053918No Hit
GCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCC1550.16001321399444599No Hit
CGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCT1520.1569161840461664No Hit
GCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAG1470.1517544674657004No Hit
CAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGT1460.15072212414960717No Hit
TCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAG1450.149689780833514No Hit
CAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGC1420.14659275088523438No Hit
AAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATC1400.14452806425304798No Hit
CGCGAACTAGCCGAGATGACGATCCCAGTCGCTGTGGTTGTCCCGAGGAG1390.14349572093695478No Hit
GGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTC1370.1414310343047684No Hit
GGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCAC1350.139366347672582No Hit
CGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCG1340.1383340043564888No Hit
GTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGG1330.1373016610403956No Hit
CGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCTGT1320.1362693177243024No Hit
GTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGA1320.1362693177243024No Hit
TTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGA1310.1352369744082092No Hit
TGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTA1280.1321399444599296No Hit
GCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGA1270.1311076011438364No Hit
GGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACC1250.12904291451165No Hit
GCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTT1250.12904291451165No Hit
GGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGG1250.12904291451165No Hit
GTTTGCCTAGCCACCCCGGTCACCCGGAATGCTGGTGCGCTCTTACCGCA1230.12697822787946358No Hit
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCG1220.12594588456337039No Hit
CGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTT1200.123881197931184No Hit
CCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACT1190.12284885461509079No Hit
CGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTA1190.12284885461509079No Hit
GTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAA1120.1156224514024384No Hit
CACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGC1090.11252542145415878No Hit
CGCGACCGGACTCATCGGTGGATCCCTCTAGCTGATGCCAGGGTCCGGGC1080.1114930781380656No Hit
GTCGAGTCGAGGAAAGTCCGGACTTCACAGAGCAGGGTGATTGCTAACGG1080.1114930781380656No Hit
TCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGAC1060.10942839150587919No Hit
CGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCG1060.10942839150587919No Hit
CGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCA1050.10839604818978599No Hit
AGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCT1030.10633136155759959No Hit
GTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTAC1020.10529901824150639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTA1050.033.2740362
TTGCCTA353.3395053E-431.194413
TTTGCCT353.3395053E-431.194412
CGGGAAT454.2424726E-529.1147821
CTCGGTT1300.028.5548821
CGGTTAC1250.027.9501913
TGCCTAG407.297301E-427.2951074
AATCGAA508.695156E-526.2033025
GTTTGCC450.001450805224.2623181
GAATGCT450.001450805224.26231827
CCTAGCC450.001450805224.2623186
AATGCTG450.001450805224.26231828
GCCTAGC450.001450805224.2623185
TTACCAA1500.023.2918246
GGTTACC1500.023.2918244
CGCGCAT3100.023.2448651
TCTTACC500.002195222522.59428441
GCGCATC3350.022.1619992
GAATCGA602.994096E-421.8360884
GGGAATC602.994096E-421.8360882
Produced by FastQC (version 0.11.3)