FastQCFastQC Report
Wed 5 Aug 2015
trimmedpyr5a.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmedpyr5a.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96615
Sequences flagged as poor quality0
Sequence length36-50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTA18491.913781503907261No Hit
CGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCG9350.967758629612379No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG7620.7886974072349013No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG7550.7814521554624023No Hit
GTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGAT6930.7172799254774104No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT6760.6996843140299126No Hit
CGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTA6230.6448274077524194No Hit
CGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACC6000.6210215804999223No Hit
CTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCGAGGAA5700.5899705014749262No Hit
ATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGT5420.5609894943849298No Hit
CAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACG4900.5071676240749367No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG4810.4978523003674378No Hit
CGGGAATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCG4570.4730114371474409No Hit
CTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCT4480.46369611343994205No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC4060.4202246028049475No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT3850.39848884748745017No Hit
CTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGC3800.39331366764995085No Hit
CAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACAT3760.38917352377995135No Hit
ATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATC3180.32914143766495885No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG3080.31879107798996015No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT2920.30223050250996225No Hit
GCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCC2360.24426848832996947No Hit
CGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCG2340.24219841639496975No Hit
GCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCG2270.23495316462247062No Hit
CCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCG2200.22770791284997152No Hit
GTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCT2200.22770791284997152No Hit
CGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGA2170.22460280494747192No Hit
CTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGG2010.208042229467474No Hit
CAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGT2000.20700719349997415No Hit
CAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGC1810.18734151011747657No Hit
CAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGG1810.18734151011747657No Hit
CGCGAACTAGCCGAGATGACGATCCCAGTCGCTGTGGTTGTCCCGAGGAG1790.18527143818247685No Hit
CTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAG1670.17285100657247837No Hit
CGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTA1650.17078093463747865No Hit
GTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTC1610.16664079076747917No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA1590.16457071883247942No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGC1560.1614656109299798No Hit
GAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCC1540.15939553899498007No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG1500.15525539512498057No Hit
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCG1490.15422035915748072No Hit
GCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAG1490.15422035915748072No Hit
CGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCTGT1480.15318532318998085No Hit
AAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATC1390.143869999482482No Hit
CTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGC1390.143869999482482No Hit
CCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACT1380.14283496351498215No Hit
GTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGA1380.14283496351498215No Hit
ATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTT1350.13972985561248255No Hit
AGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGC1340.13869481964498268No Hit
GAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCAC1300.13455467577498317No Hit
GTCGAGTCGAGGAAAGTCCGGACTTCACAGAGCAGGGTGATTGCTAACGG1280.13248460383998345No Hit
CGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCT1280.13248460383998345No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG1270.13144956787248357No Hit
TCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGAC1260.1304145319049837No Hit
GCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGA1260.1304145319049837No Hit
CCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGG1240.12834445996998395No Hit
TTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGA1220.1262743880349842No Hit
GGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCAC1180.12213424416498474No Hit
CGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCG1150.11902913626248511No Hit
CGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCT1130.11695906432748539No Hit
GTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTAC1120.11592402835998551No Hit
GACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCG1090.1128189204574859No Hit
CCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCG1080.11178388448998602No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG1080.11178388448998602No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC1080.11178388448998602No Hit
TCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGA1060.10971381255498629No Hit
GGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGAT1050.1086787765874864No Hit
GCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAA1030.10660870465248667No Hit
GTTTGCCTAGCCACCCCGGTCACCCGGAATGCTGGTGCGCTCTTACCGCA1020.1055736686849868No Hit
GGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTT1020.1055736686849868No Hit
AGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACC1010.10453863271748694No Hit
CGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCG1000.10350359674998708No Hit
CGCGACCGGACTCATCGGTGGATCCCTCTAGCTGATGCCAGGGTCCGGGC970.10039848884748745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAA850.038.5326085
TCGGGTT250.00243015834.9362342
GGGAATC900.033.9657822
CTCGGTT1200.032.752721
CGGGAAT950.032.178111
ATGAGTC353.1616603E-431.48641634
TCGGTTA1250.031.4426062
GAATCGA1050.031.1930644
ATCGAAC1100.029.7751986
TCGATCT300.00593334229.1135273
TGAGTCC406.721911E-427.68077535
TCCATCT506.37685E-527.42286143
TCGAACC1200.027.2939327
CGGTTAC1550.026.7656613
GGAATCG1150.026.5819153
TTACCAA1500.026.2021756
CTCGCGT1151.4551915E-1124.6832071
TACCAAG1600.024.5645377
GTTACCA1600.024.5645375
ACGGCTA1700.024.47506944
Produced by FastQC (version 0.11.3)