FastQCFastQC Report
Wed 5 Aug 2015
trimmedpyr5b.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmedpyr5b.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96647
Sequences flagged as poor quality0
Sequence length36-50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTA18271.890384595486668No Hit
CGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCG10911.1288503523130569No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG9660.9995136941653647No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG8330.8618994898962203No Hit
GTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGAT6840.7077301933841712No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT6660.6891057146109036No Hit
CGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACC6460.6684118493072728No Hit
CAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACG6040.6249547321696483No Hit
CGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTA5670.5866710813579314No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT5150.5328670315684916No Hit
CGGGAATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCG5080.5256241787122208No Hit
ATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGT4890.5059650066737715No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG4170.4314670915807009No Hit
CTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCGAGGAA4170.4314670915807009No Hit
GCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCC4160.4304323983155194No Hit
CTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCT3720.3849058946475317No Hit
GCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCG3580.37042018893499024No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG3510.36317733607871944No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC3500.3621426428135379No Hit
CAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACAT3500.3621426428135379No Hit
TCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGAC3290.34041408424472563No Hit
CGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGA3180.32903245832772876No Hit
CTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGC3070.31765083241073183No Hit
TTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGA3020.3124773660848241No Hit
ATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATC2830.29281819404637494No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT2820.2917835007811934No Hit
TCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGA2770.28661003445528577No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGC2560.2648814758864735No Hit
GAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCAC2410.2493610769087504No Hit
CAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGG2160.223493745279212No Hit
CGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCG2020.20900803956667044No Hit
CTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAG2010.20797334630148892No Hit
CCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCC1930.19969580018003663No Hit
TCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAG1890.19555702711931047No Hit
GCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGA1780.18417540120231357No Hit
GTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTC1760.1821060146719505No Hit
GAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCC1750.18107132140676896No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG1740.1800366281415874No Hit
CTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGG1710.1769325483460428No Hit
CCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCG1670.17279377528531667No Hit
CAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGT1650.1707243887549536No Hit
AAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATC1640.16968969548977206No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA1640.16968969548977206No Hit
CGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCG1630.1686550022245905No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG1620.16762030895940896No Hit
CGCGAACTAGCCGAGATGACGATCCCAGTCGCTGTGGTTGTCCCGAGGAG1540.15934276283795668No Hit
CCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGG1530.15830806957277516No Hit
GTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGA1500.15520398977723054No Hit
GGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTT1470.15209990998168593No Hit
CGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCG1470.15209990998168593No Hit
CAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGC1460.15106521671650439No Hit
ATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTT1450.15003052345132284No Hit
CGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCTGT1410.1458917503905967No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC1400.1448570571254152No Hit
GGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCAC1320.13657951100396287No Hit
GCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAG1320.13657951100396287No Hit
CCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACT1310.13554481773878135No Hit
GTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCT1240.12830196488251058No Hit
GTTTGCCTAGCCACCCCGGTCACCCGGAATGCTGGTGCGCTCTTACCGCA1210.12519788508696597No Hit
CGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTA1210.12519788508696597No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC1180.12209380529142136No Hit
CTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGC1180.12209380529142136No Hit
GCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTT1180.12209380529142136No Hit
TGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTA1170.12105911202623981No Hit
AGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGC1150.11898972549587675No Hit
CGCGACCGGACTCATCGGTGGATCCCTCTAGCTGATGCCAGGGTCCGGGC1140.11795503223069521No Hit
CCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCG1130.11692033896551367No Hit
GTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTAC1100.11381625916996906No Hit
TCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGT1090.11278156590478752No Hit
GTCGAGTCGAGGAAAGTCCGGACTTCACAGAGCAGGGTGATTGCTAACGG1080.111746872639606No Hit
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCG1070.11071217937442446No Hit
TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCG1060.10967748610924291No Hit
GACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCG1060.10967748610924291No Hit
GGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGAT1050.10864279284406138No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG1050.10864279284406138No Hit
GACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTA1040.10760809957887985No Hit
CGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGC1040.10760809957887985No Hit
CGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCT1020.10553871304851677No Hit
CGAGGTTCAGCGTCGAGTCGAGGAAAGTCCGGACTTCACAGAGCAGGGTG1010.10450401978333522No Hit
CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC990.10243463325297217No Hit
AGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACC970.10036524672260907No Hit
CGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAG970.10036524672260907No Hit
GCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCC970.10036524672260907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATC650.040.3072132
GAATCGA651.2732926E-1136.948284
AATCGAA651.2732926E-1136.948285
CGGGAAT751.8189894E-1234.932921
GGAATCG850.033.391763
ATCGGGG353.3421456E-431.1901076
TTCGACT701.0968506E-931.1901071
ATCGAAC801.382432E-1030.0204756
GGATCAA300.00593609229.1107654
AAGGCTC508.7030865E-526.199694
TCGGTTA1450.025.5973992
GTTACCA1550.023.9459515
CTCGGTT1550.023.9459511
CGGTTAC1600.023.1976413
TTACCAA1600.023.1976416
TACCAAG1650.022.4946827
CGGCATG500.002679347821.8330752
ATCGAGC500.002679347821.8330756
TCTCGGT803.8019316E-621.8330731
GGTTACC1750.021.2092724