FastQCFastQC Report
Wed 5 Aug 2015
trimmedpyr7b.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmedpyr7b.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96345
Sequences flagged as poor quality0
Sequence length36-50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTA13211.3711142249208572No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG8140.8448803778089159No Hit
CGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCG7030.7296694171986091No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT6310.654937983289221No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG6280.6518241735429965No Hit
CAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACG5510.5719030567232342No Hit
CGGGAATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCG4590.47641289117234936No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT4580.47537495459027457No Hit
CGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTA4560.4732990814261249No Hit
GTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGAT4030.4182884425761586No Hit
CTCGCGTAGGGCTTGTGTGGATTCACGAGGTTCAGCGTCGAGTCGAGGAA3960.4110228865016347No Hit
CGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACC3950.4099849499195599No Hit
ATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGT3650.37884685245731486No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG3510.36431574030826713No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG3240.3362914525922466No Hit
CTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGC2720.28231875032435516No Hit
CTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAGTCGCT2580.2677876381753075No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC2570.2667497015932327No Hit
CAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACAT2470.2563703357724843No Hit
CGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTCGA2260.23457366754891273No Hit
CTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTAG2230.23145985780268827No Hit
GCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCC2200.22834604805646372No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT2080.21589080907156574No Hit
CAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGG2020.20966318957911673No Hit
CTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGG1970.20447350666874253No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA1920.19928382375836834No Hit
ATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATC1880.19513207743006902No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG1770.18371477502724584No Hit
CCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCC1630.16918366287819814No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG1610.16710778971404847No Hit
GAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCC1590.1650319165498988No Hit
GCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCG1550.16088017022159945No Hit
GAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCAC1540.15984223363952463No Hit
TCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGA1530.1588042970574498No Hit
CCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCG1490.15465255072915046No Hit
CGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCG1460.15153874098292594No Hit
CCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTGCGGG1430.14842493123670145No Hit
CCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGC1360.14115937516217758No Hit
CAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGT1350.14012143858010276No Hit
CCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACT1250.1297420727593544No Hit
AGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGC1250.1297420727593544No Hit
CGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCG1220.1266282630131299No Hit
CAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGC1200.12455238984898022No Hit
ATCGAACCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTT1190.1235144532669054No Hit
GGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTT1160.12040064352068089No Hit
CGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCT1150.11936270693860605No Hit
GTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCT1110.11521096061030672No Hit
AAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATC1100.11417302402823186No Hit
GGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCAC1100.11417302402823186No Hit
TTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGA1070.11105921428200738No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC1040.10794540453578287No Hit
GTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTC1040.10794540453578287No Hit
CGCTATTCTACGCGAACTCGGCGTTGCCCTATGCGAACATCCCAGTGACG1020.10586953137163319No Hit
CGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTT990.10275572162540868No Hit
GCGAACTCGGCGTTGCCCTATGCGAACATCCCAGTGACGTTGCCTTCGGT990.10275572162540868No Hit
CGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTA990.10275572162540868No Hit
CCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCG970.10067984846125902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA558.393181E-833.77938544
CTCGGTT900.031.507041
TCGGTTA859.094947E-1230.7942092
CCAGGCT300.00553676629.52802735
GGAATCG454.273525E-529.0834223
GGGAATC454.273525E-529.0834222
CGGGAAT454.273525E-529.0834221
AATCGAA454.273525E-529.0834225
ACCCTGC300.005963323629.083425
TAAGCGA654.9031405E-727.62064438
TCGCGTA601.0293021E-525.4479922
CCCTATG450.001459880824.23618316
TATGCGA450.001459880824.23618319
GCCCTAT450.001459880824.23618315
GAATCGA551.6731452E-423.7955274
CGGTTAC1153.783498E-1022.7609373
TTACCAA1307.2759576E-1121.8125676
TGCCCTA500.00269394521.81256514
CGCGTAG703.3927303E-521.8125653
CCTATGC500.00269394521.81256517
Produced by FastQC (version 0.11.3)